Dieser Anti-PROX1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von PROX1 in WB, IF, IHC (p), EIA und IHC (fro). Geeignet für Human, Maus und Ratte.
This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by Immunostaining. This antibody cross reacts with Equine Prox-1 in Immunohistochemistry using Paraffin-Embedded Sections (Figure 2).
Aufreinigung
Antigen-Affinity Chromatography.
Immunogen
Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain).
PROX1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunofluorescence: Use 1/750-1/1000 dilution. Western Bloting: Use 1-5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL
Buffer
5 mM PBS, pH 7.2 without preservatives
Konservierungsmittel
Without preservative
Handhabung
Dilute only prior to immediate use
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Target
PROX1
(Prospero Homeobox 1 (PROX1))
Andere Bezeichnung
PROX-1
Hintergrund
Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.Synonyms: Homeobox prospero-like protein PROX1, PROX1, Prospero homeobox protein 1